Figure 1.

IGPD Catalyzes the Mn(II)-Dependent Dehydration of IGP to IAP

(A) Schematic drawing representing an overview of the reaction mechanism of IGPD, highlighting the key intermediates. Figure generated using ChemDraw.

(B) Structure-based sequence alignment showing equivalent residues in A. thaliana IGPD2 (ARATHA2) and IGPD1 (ARATHA1). Black and gray shading indicates identical or similar residues, respectively. Secondary structure elements are numbered and represented as gray arrows (beta strands), tubes (helices), and lines (loop regions) above the sequence. The C loop is labeled and shown as a gray line. Black dots indicate residues that are involved in metal ion binding. Residues implicated in recognition of the substrate-phosphate are marked with a black triangle. Numbering is based on the convention adopted in the structure of IGPD1 (PDB: 2F1D) (Glynn et al., 2005). Sequences were retrieved from UniProt, the alignment was produced using Tcoffee, and the figure was drawn using boxshade. See also Figure S1.