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. 2015 Jul 7;23(7):1236–1245. doi: 10.1016/j.str.2015.05.012

Figure 4.

Figure 4

A Comparison of the Two Phosphate Binding Sites Observed in the Form A and Form B Complexes

(A) The interactions between the phosphate group of the IGP (green carbons) and the enzyme in form A. Hydrogen bonds are shown as orange dashes. Representation of the metal ion, backbone, and side-chains colors is the same as in Figures 3A and 3B.

(B) The interactions between the phosphate group of the IGP (yellow carbons) and the enzyme in form B. Hydrogen bonds are drawn as orange dashes. HOH5 is labeled and shown as a red sphere. Representation of the metal ion, backbone, and side-chains colors, including the red C loop, is the same as in Figures 3D and 3E.

(C) A superposition of the structures shows that the ordering of the C loop (red) in form B repositions the phosphate group into a site that is ∼2.2 Å away from that in form A (double-headed arrow). There are also minor differences in the conformation of the side chains surrounding the two sites.