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. Author manuscript; available in PMC: 2015 Jul 21.
Published in final edited form as: Biochim Biophys Acta. 2012 Sep 6;1823(12):2179–2189. doi: 10.1016/j.bbamcr.2012.08.021

Fig. 3.

Fig. 3

AGE-BSA induced ER stress is mediated through RAGE in human chondrocytes. (A) RAGE-specific siRNA transfection on AGE-BSA-induced expression of GRP78 and COX-2 in OA chondrocytes. OA chondrocytes were transfected with RAGE-siRNA or control siRNA and then stimulated with AGE-BSA for 24 h. (A) Expression of GRP78 and COX-2 mRNA was determined as described under Fig. 1. (B) Protein expression of RAGE, GRP78 and COX-2 was determined by Immunoblot analysis. β-actin was used as a loading control. Production of PGE2 was quantified by ELISA. Immunoblots are from single chondrocytes sample, but are representative of five chondrocytes samples. Bars show the mean with 95% CI results of five independent assays, each of which run in triplicate experiments. #p<0.05 versus chondrocytes treated with AGE-BSA+control siRNA; *p<0.001 versus chondrocytes transfected with control siRNA alone.