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. 2015 Jul 21;10(7):e0133619. doi: 10.1371/journal.pone.0133619

Fig 3. Generation of TgCC10Enpp2 mice.

Fig 3

A. Schematic representation of the construct used for the generation of the transgenic mice. B. Genotyping PCR of the 4 offsprings that carried the transgene, out of the 54 that were generated after the injections of the trangene-microinjected zygotes in surrogate mothers. C. All four transgenic lines contained equal copy numbers, as identified with Real-Time PCR. D. Real-Time RT-PCR confirmed the expression of the transgene (L39 is shown). E. Total ATX activity levels in the BALFs of TgCC10Enpp2 mice (L39) were found moderately upregulated with the TOOS assay. F. In the same mice, BALF LPA was also found elevated, as measured with HPLC-MS/MS. (C-F n = 3–8).