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. 2015 Jul 21;10(7):e0133217. doi: 10.1371/journal.pone.0133217

Fig 3. Extracellular sialidase activity of wild-type C. perfringens, BT1 (nanI), BT2 (nanJ), and BT3 (nanInanJ).

Fig 3

Cells were grown overnight in 5 ml of PY with (+) or without (-) 1 mg/ml sialic acid as indicated. Sialidase specific activity was defined as μmoles of 4-methylumbelliferyl-α-D-N-acetylneuraminic acid hydrolyzed per minute per milligram of protein. Values shown are the mean and SEM of triplicate biological samples. The P values, calculated using the student's two-tailed t-test, are shown in the figure.