Fig. 2.

Aberrant expression of miR-325 regulates HCC cell lines invasion. a Expression of miR-325 was analyzed in five liver cancer cell lines (SMMC-7721, Hep3B, HepG2, Huh7 and Bel7404) and normal liver tissue (LO2). b The results of miR-325 expression in cell lines transfected with miR-325 mimics, miR-325 inhibitor, control for miR-mimics (NC) and control for miR-325 inhibitor (inhibitor NC) were validated by using qRT-PCR. c Transwell assay was performed according to the Material and Methods. HepG2 and Huh7 were treated with miR-325 mimics, miR-325 inhibitor, NC and inhibitor NC for 24 h. The representative images of invasive cells at the bottom of the membrane stained with crystal violet were visualized as shown. The quantifications of cell invasion were presented as percentage of cell numbers. Data are shown as mean ± SEM of three independent experiments. * indicates significant difference compared with control group (P < 0.05)