(A) Crystal structure of MAGE-G1-NSE1 complex. MAGE-G1 MHD forms a tandem winged helix (WH-A, purple; WH-B, cyan). The two zinc ions coordinated by the NSE1 RING domain are shown as gray spheres.
(B) Crystal structure of the MAGE-A4 MAGE homology domain (PDB: 2WA0) consisting of two winged helix motifs (WH-A, yellow-green; WH-B, cream).
(C) Orientation of MAGE WH-A to WH-B differs between MAGE-G1 and MAGE-A4. (Left) MAGE-A4 aligned to MAGE-G1-NSE1 based on WH-A motif. (Right) MAGE-A4 WH-B aligns with MAGE-G1 WH-B after rotation, as indicated by the arrow.