Multiplexed drug-specific p-BNC panels recorded with the ‘macro’ laboratory prototype. (A) Panel for drugs cocaine, methamphetamine, amphetamine and diazepam; (B) demonstration of the specificity of tracers for their respective drug targets tetrahydrocannabinol (THC), temazepam, methamphetamine, morphine and methadone; (C) quantitative demonstration of specificity of duplex p-BNC assay for the drugs methadone and morphine with evidence of dose dependent reduction of signal, i.e. competition.
CAL: beads coupled to a fixed amount of fluor serve as calibrators of the p-BNC system
CTL: beads coupled to bovine serum albumin alone, serve as negative controls and further indicators of the specificity of the reactions that take place within the flow cell.