Figure 3.

Serum proteins adsorb to wetted TIPS microspheres in a dose-dependent manner. PLGA microspheres were suspended in medium containing 0%, 2%, 10% or 20% FBS and treated 70% (v/v) ethanol (EtOH), for 30 min, 3 h and 24 h (a). Arrows and image insets show detailed images of submerged microspheres. The total amount of serum protein adsorbed to microspheres was measured after each incubation period (b). Data points represent the mean (n = 3 ± SEM) amount of total protein. *p ≤ 0.05 indicates differences between wetting conditions. Images represent three individual experiments.