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. 2015 Aug;21(8):1444–1453. doi: 10.1261/rna.049601.115

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© 2015 Simon et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 2. — (A,C) ¹³C–¹H correlation of the base (A) and ribose (C) region of the SOLE RNA at 308 K. All resonances are visible in the spectra. (B,D) ¹³C–¹H correlation of the base (B) and ribose (D) region of the SOLE RNA at 283 K. Only the resonances of the first 5–6 bp from the termini are visible. All other resonances are exchange-broadened beyond detection. (E) Ratio of transverse and longitudinal relaxation rates of C6/C8 atoms in dependence of the radio frequency field strength B₁ of the R_1ρ spinlock pulse (given in Hertz in the inset). For the two smallest B1 fields, only residues with resonance offsets smaller than 76 Hz are shown. (F) Order parameters S² (dots) and exchange contributions R_ex (bars) characterizing the internal motions of the RNA. The green bars show the R_ex contribution averaged over all carbon atoms of the corresponding residue. The order parameters are indicated by colored dots, differentiating the different carbon atoms. The black line connects the S² values averaged of all carbons of each residue.