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. 2015 Aug;21(8):1454–1468. doi: 10.1261/rna.051383.115

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© 2015 Nord et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 7. — The activity of K529 mutants of firefly luciferase (Fluc) expressed in strains carrying various rpsE suppressor mutations in (A) the wild-type background or (B) in the presence of the rbfA::Km^R mutation. The Fluc activities produced for each of the mutants of Fluc K529 are expressed as a ratio to that of the wild-type enzyme. Activities are shown for the four known error-prone mutants replacing the wild-type Lys codon with AAU (Asn), UAG (stop), AGG (Arg), or AGA (Arg). Error bars are standard errors of the mean. Significance is indicated: (*) P ≤ 0.05; (**) P ≤ 0.005.