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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Anesthesiology. 2015 Aug;123(2):377–388. doi: 10.1097/ALN.0000000000000742

Figure 4. Isoflurane prevents increased alveolar-capillary barrier permeability following injury with lipopolysaccharide and mechanical ventilation.

Figure 4

C57BL/6 mice were subjected to injury with nebulized lipopolysaccharide (LPS) and mechanical ventilation to induce ventilator induced lung injury (LPS/VILI) or nebulized phosphate buffered saline followed by mechanical ventilation (PBS/VILI) prior to performing bronchoalveolar lavage (BAL). A. BAL protein increased significantly following LPS/VILI (n=3) compared to PBS/VILI (n=3). Isoflurane treatment following LPS but prior to VILI (n=4) prevented the increase in BAL protein seen with LPS/VILI without isoflurane (n=3). (*p<0.001 vs LPS/VILI no isoflurane by 2-way ANOVA with Bonferroni post-test). In a separate experiment, mice were subjected to LPS/VILI with isoflurane (n=6) or control gas (n=5). Mice treated with isoflurane had significantly less alveolar-capillary permeability as measured by Evan blue assay (B) and wet to dry ratio (C). **p<0.05 vs LPS/VILI no isoflurane by Mann-Whitney test.