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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Anesthesiology. 2015 Aug;123(2):377–388. doi: 10.1097/ALN.0000000000000742

Figure 6. Isoflurane exposure attenuates the reduction in lung epithelial zona occludens 1 after injury with lipopolysaccharide and mechanical ventilation in vivo.

Figure 6

Wild-type C57BL/6 mice (n=3/group) were subjected to lung injury with the combination of lipopolysaccharide followed by mechanical ventilation to induce ventilator induced lung injury (LPS/VILI) with or without isoflurane treatment and compared to control mice subjected to treatment with nebulized phosphate buffered saline (PBS) without VILI. Zona occludens 1 (ZO-1) immunostaining revealed decreased ZO-1 expression in LPS/VILI (B) that was discontinuous in nature compared to control animals (A). The addition of isoflurane treatment after LPS but prior to mechanical ventilation (C) restored ZO-1 levels back to those seen in control mice, and staining was smooth and continuous in nature similar to that seen in control mice. Quantitation of ZO-1 staining (D, 3 images per mouse) from lung sections confirmed that ZO-1 levels significantly decreased with LPS/VILI and that isoflurane treatment prevented this decrease. *p<0.05 vs all other groups by 1-way ANOVA with Tukey’s multiple comparisons test.