Table 1.

Summary of detailed lung physiology data presented in Figure 2.

	PBS/VILI				LPS/VILI
	control		isoflurane		control		isoflurane
	G	H	G	H	G	H	G	H
baseline	6.12 ± 0.12	28.20 ± 0.80	6.02 ± 0.23	28.5 ± 0.82	6.24 ± 0.28	30.2 ± 1.35	5.31 ± 0.27	25.2 ± 1.48
30 min	5.98 ± 0.23	27.80 ± 0.67	5.78 ± 0.21	27.0 ± 0.51	6.56 ± 0.25	32.0 ± 1.56	5.16 ± 0.31	26.5 ± 1.35
60 min	5.75 ± 0.23	27.90 ± 0.63	5.67 ± 0.22	27.1 ± 0.53	6.65 ± 0.31	32.6 ± 1.83	5.31 ± 0.29	27.0 ± 1.22
90 min	5.85 ± 0.21	28.00 ± 0.70	5.42 ± 0.26	26.1 ± 1.77	6.80 ± 0.40	34.3 ± 2.26	5.59 ± 0.42	28.0 ± 1.44
120 min	5.67 ± 0.31	27.90 ± 0.94	5.68 ± 0.20	27.5 ± 0.70	6.98 ± 0.37	34.4** ± 2.30	5.64* ± 0.44	28.1* ± 1.69

C57BL/6 mice (n=8/group) were treated with nebulized lipopolysaccharide (or saline control) 1 hr prior to exposure to isoflurane (or control gas) 24 hrs prior to mechanical ventilation. Lung elastance (i.e. stiffness, H, cm H₂O/mL) and tissue resistance (G, cm H₂O/mL) were measured at the onset of mechanical ventilation (i.e. baseline) and every 30 min thereafter. Following 2 hrs of mechanical ventilation, mice injured with lipopolysaccharide (LPS/VILI) had significantly higher lung elastance (H) compared to all other groups

^**p<0.05 vs PBS, PBS + iso, LPS + iso by 1-way ANOVA with Tukey’s multiple comparisons test.

At 2 hrs, LPS/VILI mice treated with isoflurane had significantly lower elastance (H) and resistance (G) compared to LPS/VILI mice without isoflurane treatment

^*p<0.05 by 1-way ANOVA with Tukey’s multiple comparisons test.

G = lung tissue resistance; H = lung elastance (stiffness); LPS/VILI = mechanically ventilated mice previously treated with nebulized endotoxin; PBS/VILI = mechanically ventilated mice previously treated with nebulized phosphate buffered saline