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Primary antibodies and dilutions used in this study.
| Antigen | Primary antibody | Dilution |
|---|---|---|
| CD3 (pan T cell marker) | DAKO M7254 (F7.2.38) | 1:60 |
| CD4 (helper T cell) | DAKO M7310 (4B12) | 1:40 |
| CD8 (cytotoxic T cell) | DAKO M7103 (C8/144B) | 1:100 |
| CD20 (B cell) | DAKO M0755 (L26) | 1:200 |
| CD68 (macrophage) | DAKO M0718 (EBM11) | 1:100 |
| CD11c (M1 marker) | Abcam Ab-53441 | 1:100 |
| CD163 (M2 marker) | Santa Cruz SC-20066 | 1:100 |
| CD123 (PDC marker) | Novus NB-600-1185 | 1:100 |
