Table 2. Presence of M. ulcerans in aquatics vertebrates and macro-invertebrates collected in different areas of Oueme.
| Mitro | Houeda | Agonhoui | Agbomahan | Agbonan | Assigui | Kode | Gbame | Bonou | Total | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vertebrates | Fish | 17 (0/5) | 1 (0/1) | 54 (0/6) | - | 2 (0/2) | 88 (1/5) | 3 (0/1) | 3 (0/2) | - | 169 (1/22) | |
| Anura | 6 (0/4) | 12 (1/4) | 19 (0/4) | 14 (0/2) | 79 (0/3) | 18 (1/3) | 2 (0/1) | 2 (0/1) | 12 (0/1) | 164 (2/24) | ||
| Invertebrates | Insecta | Odonata | 36 (0/5) | 5 (0/3) | 17 (1/4) | - | 44 (0/4) | 10 (0/4) | 2 (0/3) | 6 (0/2) | 22 (0/1) | 142 (1/26) |
| Hemiptera | 676 (2/36) | 216 (1/23) | 22 (2/12) | 6 (0/3) | 269 (1/17) | 59 (3/13) | 111 (1/15) | 122 (0/15) | 109 (0/7) | 1590 (10/141) | ||
| Coleoptera | 288 (2/17) | 118 (2/8) | 1 (0/1) | - | 6 (0/2) | 32 (0/5) | 75 (1/9) | 53 (0/7) | 27 (1/4) | 600 (6/53) | ||
| Diptera | - | - | - | - | 100 (1/2) | - | - | 1 (0/1) | 1 (0/1) | 102 (1/4) | ||
| Mollusca | 100 (0/4) | 16 (0/2) | 148 (0/6) | - | - | 1 (0/1) | 3 (0/1) | - | - | 268 (0/14) | ||
| Crustacea | Decapoda | 5 (0/1) | - | 93 (2/9) | 59 (0/5) | - | 150 (5/11) | 1 (0/1) | - | - | 308 (7/27) | |
| Araneae | 7 (0/1) | 3 (0/2) | 18 (0/4) | - | 1 (0/1) | 2 (0/1) | 3 (0/1) | 1 (0/1) | - | 35 (0/11) | ||
| Total | 1135 (4/73) | 371 (4/43) | 372 (5/46) | 79 (0/10) | 501 (2/31) | 360 (10/40) | 200 (2/32) | 189 (0/29) | 171 (1/14) | 3378 (28/322) |
The figures correspond to the abundance and the figures in brackets correspond to the number of positive qPCR pools (number of positive pools/total pools). qPCR targeting the KR and IS2404 sequences was used to detect M. ulcerans. Only sample pools testing positive for both sequences were considered positive.