Fig 3. GPR18 is essential for optimal IEL reconstitution following bone marrow transplantation.

Donor chimerism analysis of T cells in the intestine and spleen. Equal numbers of wild type CD45.1⁺ and either Gpr18 ^+/+ or Gpr18 ^-/- CD45.2⁺ bone marrow cells were transplanted into 800cGy-irradiated CD45.1⁺ recipients. Eight weeks after reconstitution, small (A, C) or both small and large (B) intestines were harvested and donor CD45.2⁺ chimerism in TCRγδ and TCRαβ (A, B) and specific IEL subsets (C) were analyzed by flow cytometry. (D) Total splenic T cell chimerism is shown. Gating strategies are shown in S1 Fig. Mean values ± SEM are shown. *p<0.05 using student's 2-tailed unpaired t-test.