Fig 5. GPR18 is dispensable for resistance to C. rodentium infections and graft versus host disease.

(A) 5 x 10⁶ bone marrow cells from Gpr18 ^-/- or Gpr18 ^+/+ mice were transplanted into sublethally irradiated wild type CD45.1 recipients. Intestinal IEL chimerism was measured 8 weeks post-transplantation. Mean values ± SEM are shown. *p<0.05 using student's 2-tailed unpaired t-test. (B) Gpr18 ^-/- or Gpr18 ^+/+ bone marrow chimeras were orally gavaged with C. rodentium. Weight after infection was quantified for 37 days. Mean values ± SEM are shown, n = 10 for each group. No statistically significant differences were observed at any timepoint by students' 2-tailed t test. (C) Lethally-irradiated (900 cGy) Balb/c mice were transplanted with 5 x 10⁶ T cell-depleted bone marrow cells from a CD45.1⁺ C57Bl/6 mice with or without 5 x 10⁵ splenic pan T cells from CD45.2⁺ Gpr18 ^+/+ or Gpr18 ^-/- littermates. Survival was measured over the course of 50 days. Two recipients of T cell-depleted bone marrow only, 10 recipients of Gpr18 ^-/- splenic pan T cells, and 5 recipients of Gpr18 ^+/+ splenic pan T cells were assessed. No significant differences (p = 0.49) in survival were observed using log-rank Mantel-Cox test.