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. Author manuscript; available in PMC: 2015 Jul 22.
Published in final edited form as: Methods Mol Biol. 2013;981:193–200. doi: 10.1007/978-1-62703-305-3_15

Figure 1.

Figure 1

Coomassie stained SDS-PAGE gel showing induction of the NatB complex and a target protein. Once a fresh culture of BL21-DE3-pNatB pTarget cells had reached an OD595nm of 0.4, IPTG was added to a final concentration 100 μg/ml. Samples of cells were taken at 0, 1, and 2 hours from addition of IPTG, boiled in SDS buffer and run on an SDS-PAGE gel. Intense protein bands can be observed in the each of the post induction samples which have migrated with masses corresponding to the NatB catalytic subunit, Naa20 (20.5 kDa), the NatB regulatory subunit, Naa25 (92.4 kDa) and the target protein.