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. Author manuscript; available in PMC: 2015 Jul 22.
Published in final edited form as: Mol Membr Biol. 2008 Feb;25(2):115–127. doi: 10.1080/09687680701633257

Figure 4.

Figure 4

Extent of activation was dependent on the modifying reagent. Hela Cells expressing Q111C or N112C mutants were first treated with 1 mM MTSEA (EA), MTSET (ET) or MTSES (ES) for 10 min and the cells were washed to remove the unreacted MTS-reagents. The washed cells were further treated with a second MTS-reagent as indicated and then assayed for 5-HT influx. Data are expressed as a percentage of the untreated activity of Q111C (A) or N112C (B). (The figure shows results of a typical experiment that was replicated three times with similar results.)