TABLE 2.

Primers and annealing temperature used for MLVA

Locus	Primer	Primer sequence (5′-3′)	Annealing temp (°C)
V2	V2F	CATCACTTCCTTGTTAAG	50
	V2R	CAATGTCCGTGATTATACA
V6	V6F	GCAAGCTCATCAAGACTTT	55
	V6R	CTTTCYACCTCATTGCTATAA
V11	V11F	ATGYCCTATGGTTCTACTTAG	55
	V11R	GCAGGCTTTGCCACT
V13	V13F	TCAAGTAGAGTTTGTATTAGAACTTG	55
	V13R	TAACAATGTCCGTGATTATACA
V17	V17F	CTCGTATTTATCCGCC	50
	V17R	TCATCTAACTCTTGACGC
V19	V19F	TCCAAAAGGTTAAAAGCCT	55
	V19R	TGAAACGCATTATCTTACTATCTAG
V33	V33F	TCAAACCAAGGATATTGTAATAAT	55
	V33R	CTGCTGATAATTTACCAAATGT