TABLE 1.
Activities of itaconate, (S)-citramalate, mesaconate, and citraconate conversions into acetyl-CoA and pyruvate in extracts of B. xenovorans cells grown on different substrates
| Reaction substrates | Activity (nmol min−1 mg protein−1) on growth substratea: |
||
|---|---|---|---|
| Acetate | Itaconate | Mesaconate | |
| Itaconate + succinyl-CoA | 1.0 ± 0.4 | 266 ± 1 | 11 ± 2 |
| (S)-Citramalate + succinyl-CoA | 1.3 ± 0.4 | 396 ± 18 | 224 ± 1 |
| Mesaconate + succinyl-CoA | <1 | 35 ± 5 | 76 ± 5 |
| Citraconate + succinyl-CoA | <1 | <1 | <1 |
| Itaconate + ATP + CoA | 2 ± 0.9 | 109 ± 22 | 17 ± 1 |
| (S)-Citramalate + ATP + CoA | <1 | <1 | <1 |
| Mesaconate + ATP + CoA | <1 | <1 | <1 |
a
The activities were measured spectrophotometrically following pyruvate formation with phenylhydrazine. Values are means ± standard deviations of results from at least two independent measurements.