TABLE 2.

Activities of enzymes involved in itaconate, (S)-citramalate, and mesaconate degradation in B. xenovorans cells grown on different substrates

Enzyme	Activity (nmol min−1 mg protein−1) on growth substratea:
	Acetate	Itaconate	Mesaconate
Succinyl-CoA:itaconate-CoA transferase	26 ± 2	358 ± 68	168 ± 15
Itaconyl-CoA hydratase	2 ± 1	229 ± 42	13 ± 3
(S)-Citramalyl-CoA lyase	8 ± 2	680 ± 70	306 ± 52
Succinyl-CoA:(S)-citramalate-CoA transferase	<1	355 ± 98	181 ± 22
Fumarase	1,480 ± 140	290 ± 100	723 ± 83
Mesaconase	472 ± 46	113 ± 24	214 ± 27

^aValues are means ± standard deviation of results from at least two independent measurements.