TABLE 2.
Enzyme | Activity (nmol min−1 mg protein−1) on growth substratea: |
||
---|---|---|---|
Acetate | Itaconate | Mesaconate | |
Succinyl-CoA:itaconate-CoA transferase | 26 ± 2 | 358 ± 68 | 168 ± 15 |
Itaconyl-CoA hydratase | 2 ± 1 | 229 ± 42 | 13 ± 3 |
(S)-Citramalyl-CoA lyase | 8 ± 2 | 680 ± 70 | 306 ± 52 |
Succinyl-CoA:(S)-citramalate-CoA transferase | <1 | 355 ± 98 | 181 ± 22 |
Fumarase | 1,480 ± 140 | 290 ± 100 | 723 ± 83 |
Mesaconase | 472 ± 46 | 113 ± 24 | 214 ± 27 |
Values are means ± standard deviation of results from at least two independent measurements.