TABLE 3.
Enzyme | Substrate | Vmax (U mg protein−1) | Km (mM) | kcat/Km (M−1 s−1) |
---|---|---|---|---|
Class I fumarase (Bxe_A3136) | Fumarate | 296 ± 5 | 0.1 ± 0.01 | 2.8 × 106 |
(S)-Malate | 118 ± 3 | 0.28 ± 0.02 | 3.98 × 105 | |
Mesaconate | 117 ± 6 | 0.03 ± 0.005 | 3.6 × 106 | |
(S)-Citramalate | 74 ± 2 | 0.52 ± 0.05 | 1.31 × 105 | |
Class II fumarase (Bxe_A1038) | Fumarate | 376 ± 12 | 0.138 ± 0.013 | 2.2 × 106 |
(S)-Malate | 165 ± 6 | 0.42 ± 0.06 | 3.2 × 105 | |
Mesaconate | <0.1 | |||
(S)-Citramalate | <0.1 |
Values are means ± standard deviation of results from at least three measurements.