TABLE 1.
Identification of bacteria inducing nodules on soybean trap plants grown in the field locations shown in Fig. 1
| Location | No. of nodules analyzed | No. (%) of nodules containing strain: |
|
|---|---|---|---|
| USDA 110a | USDA 123b | ||
| 1N | 10 | 6 (60) | 4 (40) |
| 2N | 9 | 6 (66.7) | 3 (33.3) |
| 3N | 5 | 2 (40) | 3 (60) |
| 4N | 3 | 2 (66.7) | 1 (33.3) |
| 5N | 0 | ||
| Total | 27 | 16 | 11 |
| 1S | 9 | 0 | 9 (100) |
| 2S | 9 | 3 (33.3) | 6 (66.7) |
| 3S | 9 | 8 (88.9) | 1 (11.1) |
| 4S | 9 | 3 (33.3) | 6 (66.7) |
| 5S | 9 | 2(22.2) | 7(77.8) |
| 6S | 9 | 5 (55.5) | 4 (44.5) |
| 7S | 9 | 3 (33.3) | 6 (66.7) |
| 8S | 9 | 8 (88.9) | 1 (11.1) |
| 9S | 9 | 7 (77.8) | 2 (22.2) |
| Total | 81 | 39 | 42 |
| 1W | NDc | ||
| 2W | 9 | 6 (66.7) | 3 (33.3) |
| 3W | 9 | 7 (77.8) | 2 (22.2) |
| 4W | 9 | 7 (77.8) | 2 (22.2) |
| 5W | 6 | 2 (33.3) | 4 (66.7) |
| 6W | 9 | 5 (55.5) | 4 (44.5) |
| 7W | 1 | 1 | 0 |
| 8W | 3 | 2 | 1 |
| 9W | 0 | ||
| 10W–22W | 0 | ||
| Total | 46 | 33 | 16 |
| 1E | 9 | 4 (44.5) | 5 (55.5) |
| 2E | 9 | 1 (11.1) | 8 (88.9) |
| 3E | 9 | 1 (11.1) | 8 (88.9) |
| 4E | 9 | 3 (33.3) | 6 (66.7) |
| 5E | 9 | 2 (22.2) | 7 (77.8) |
| 6E | 9 | 8 (88.9) | 1 (11.1) |
| 7E | 9 | 8 (88.9) | 1 (11.1) |
| 8E | 9 | 9 (100) | 0 (0) |
| 9E | 9 | 9 (100) | 0 (0) |
| Total | 81 | 45 | 36 |
a
Identification is based on PCR analyses done using USDA 110-specific primers (Bj110F and Bj110R).
b
Bacteria which did not reveal the presence of a USDA 110-specific amplicon. The nucleotide sequences of recA from five randomly picked isolates were determined, showing their identity with USDA 123.
c
ND, not done (no plants germinated in this location).