TABLE 1.
Strains, plasmids, and oligonucleotides used in this study
| Strain, plasmid, or oligonucleotide | Descriptiona | Source or referenceb |
|---|---|---|
| Strains | ||
| B. subtilis | ||
| B15029 | Wild type (Ent+) | DSM 15029 |
| B6633 | Wild type (Sub+) | ATCC 6633 |
| B6633.MB1 | ΔspaS amyE::PspaS-lacZ (Specr Cmr Sub−) | 31 |
| PDC204 | W168 trpC2 ΩspoVD::Erm Pxyl-murE (Eryr) | 34 |
| B168.AUT3 | PDC204 pyrD::Kanr PspaRK-spaRK (Eryr Kanr) | This work |
| B168.AUT5 | B168.AUT3 thrC::Specr PspaS-lacZ (Eryr Kanr Specr) | This work |
| E. coli DH5α | recA1 endA1 gyrA96 thi hsdR17(rK− mK+) relA1 supE44 ϕ80ΔlacZΔM15 Δ(lacZYA-argF)U169 | Laboratory stock |
| Plasmids | ||
| pXT | bla thrC′ Spec ′thrC | 35 |
| pPyr-kan | bla pyrD′ Kan ′pyrD | 36 |
| pSB5 | bla amyE′ PspaS-lacZ Cm ′amyE | 20 |
| pAUT1 | pXT PspaS-lacZ | This work |
| pAUT2 | pPyr-kan PspaRK-spaRK | This work |
| Oligonucleotides | ||
| AUT1 | CGATCAATTGAATTCTGGAAGG | This work |
| AUT2 | TAGCAAGCTTTCACGCAGAATTAAAT | This work |
| AUT3 | GCATGGATCCGCATGAAATAAATTCAGGGGTATTG | This work |
| AUT4 | GCTAGAGCTCGAAGATGGATCAGTTCAC | This work |
a
Ent+, entianin producer; Sub+, subtilin producer; Cmr, chloramphenicol resistant; Specr, spectinomycin resistant; Kanr, kanamycin resistant; Eryr, erythromycin resistant.
b
DSM, German Resource Centre for Biological Material; ATCC, American Type Culture Collection.