TABLE 1.

Bacterial strains, primers, and plasmids used in this study

Strain name	Genotype or descriptiona	Source or sequence
Strains
Clostridium phytofermentans ISDg	ATCC type strain 700394	Susan Leschine Laboratory, University of Massachusetts, Amherst, Amherst, MA, USA
E. coli Top 10	hsdR mcrA endA1 recA1 rpsL (Strr) (cloning strain)	Invitrogen Corporation
E. coli S17-1	RP4-2 (Km::Tn7 Tc::Mu-1) recA1 endA1 (conjugal strain)	Yale E. coli Stock Center
Primers
pdcAdhB_F	Forward primer for amplification of pdc and adhB from pES120	5′-TTTTTCGAATTCACCGGATCCCTGCAGTAGGAGGAATTAACC-3′
pdcAdhB_R	Reverse primer for amplification of pdc and adhB from pES120	5′-ATATTTCGATCGATTGCATGCTTAGAAAGCGCTCAGGAAGAG-3′
pQexp_F	Forward primer to confirm pdc-adhB insertion in pQexp	5′-AAACCTAGGTAATTGAGGAAAGTTACAATTA-3′
pQexp_R	Reverse primer to confirm pdc-adhB insertion in pQexp	5′-GAATGGCGCCTGATGCG-3′
cphy3925F	Forward primer to amplify Cphy3925 coding sequence	5′-AAAGAAGGAGATAGGATCATGACGAAGAAAGTGGAATTA-3′
cphy3925R	Reverse primer to amplify Cphy3925 coding sequence	5′-GTGTAATGGATAGTGATCTTAATGGTGATGGTGATGATGTTTACCGTAGTACACTTTAAGATAG-3′
Plasmids
pES120	Source of Z. mobilis pdc and adhB genes	Jay Keasling Laboratory, University of California, Berkeley, Berkeley, CA, USA
pQexp	Replicating plasmid for C. phytofermentans	Andrew Tolonen Laboratory, Genoscope-CEA, Évry, France
pQexpE	pQexp with Z. mobilis pdc and adhB cloned into the unique BamHI and PvuI sites	This study

^aStr, streptomycin, Km, kanamycin; Tc, tetracycline.