FIG 3.

Mutation of the mcpP gene does not alter motility but reduces chemotaxis to pyruvate and propionate. (A) Swim plate assays of P. putida KT2440R and its mcpP-deficient mutant. Shown are representative images. (B) Plate gradient chemotaxis assays in which 10 mM pyruvate or propionate were deposited along a vertical line on MS agar plates. After overnight incubation for gradient formation, suspensions of wt or mcpP mutant strains were deposited at either side. Plates were inspected the following day for chemotaxis. This assay was repeated 3 times, and representative images are shown. To visualize the acentric spread, thin vertical lines that go through the center of bacterial deposition are shown.