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. 2015 Jul 21;81(16):5449–5457. doi: 10.1128/AEM.01529-15

TABLE 1.

Bacterial strains and plasmids used

Strain or plasmid Relevant genotype and/or descriptiona Source or reference
Escherichia coli
    BL21(DE3) F ompI hsdSB (rB mB) gal dam met 49
    DH5α F endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17 (rK mK+) phoA supE44 λ; host for DNA manipulations 50
    TK1132 Apr; E. coli DH5α derivative carrying plasmid pJRG1 This work
    CC118 λpir Rifr; Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 Tn7 λpir 51
    TK1133 Rifr Smr; E. coli CC118 λpir derivative carrying pJRG2 This work
    HB101 Smr; K-12/B hybrid; recA thi pro leu hsdRM with pRK600 53, 54
Pseudomonas putida
    KT2440R Rifr derivative of P. putida KT2440, wild type 52
    TK1134 Rifr Apr; P. putida KT2440R Δpp2861 This work
P. fluorescens KU-7 Wild type, grows on 2-nitrobenzoate, chemotactic to 2-nitrobenzoate 55
Plasmids
    pGEMT Apr; cloning vector Promega
    pUC18NotI Apr; similar to pUC18 but with NotI sites flanking the multiple-cloning site 51
    pJRG1 pUC18NotI derivative containing a 1-kb HindIII-EcoRI fragment from the P. putida KT2440R genome (containing upstream and downstream regions of pp2861) This work
    pRK600 Cmr; ori of ColE1; RK2-mob+ RK2-tra+; conjugational helper plasmid 54
    pKNG101 Smr; suicide vector; mob sac 56
    pJRG2 Smr; pKNG101 derivative containing a 1.1-kb NotI fragment from pJRG1 cloned into pKNG101 This work
    pET28b Kmr; protein expression plasmid Novagen
    pET28b-McpP-LBD Kmr; pET28b containing McpP LBD This work
    pET28b-NbaY-LBD Kmr; pET28b containing NbaY LBD This work
    pBBR1MCS2_START Kmr; oriRK2 mobRK2 57
    pMAMV240 Kmr; a 2.1-kb PCR fragment containing the mcpP gene and its promoter region was cloned into the HindIII/XbaI sites of pBBR1MCS2-START This work
a

Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin; Rif, rifampin; Sm, streptomycin.