TABLE 1.
Strain or plasmid | Relevant genotype and/or descriptiona | Source or reference |
---|---|---|
Escherichia coli | ||
BL21(DE3) | F− ompI hsdSB (rB− mB−) gal dam met | 49 |
DH5α | F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG ϕ80dlacZΔM15 Δ(lacZYA-argF)U169 hsdR17 (rK− mK+) phoA supE44 λ−; host for DNA manipulations | 50 |
TK1132 | Apr; E. coli DH5α derivative carrying plasmid pJRG1 | This work |
CC118 λpir | Rifr; Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 Tn7 λpir | 51 |
TK1133 | Rifr Smr; E. coli CC118 λpir derivative carrying pJRG2 | This work |
HB101 | Smr; K-12/B hybrid; recA thi pro leu hsdRM with pRK600 | 53, 54 |
Pseudomonas putida | ||
KT2440R | Rifr derivative of P. putida KT2440, wild type | 52 |
TK1134 | Rifr Apr; P. putida KT2440R Δpp2861 | This work |
P. fluorescens KU-7 | Wild type, grows on 2-nitrobenzoate, chemotactic to 2-nitrobenzoate | 55 |
Plasmids | ||
pGEMT | Apr; cloning vector | Promega |
pUC18NotI | Apr; similar to pUC18 but with NotI sites flanking the multiple-cloning site | 51 |
pJRG1 | pUC18NotI derivative containing a 1-kb HindIII-EcoRI fragment from the P. putida KT2440R genome (containing upstream and downstream regions of pp2861) | This work |
pRK600 | Cmr; ori of ColE1; RK2-mob+ RK2-tra+; conjugational helper plasmid | 54 |
pKNG101 | Smr; suicide vector; mob sac | 56 |
pJRG2 | Smr; pKNG101 derivative containing a 1.1-kb NotI fragment from pJRG1 cloned into pKNG101 | This work |
pET28b | Kmr; protein expression plasmid | Novagen |
pET28b-McpP-LBD | Kmr; pET28b containing McpP LBD | This work |
pET28b-NbaY-LBD | Kmr; pET28b containing NbaY LBD | This work |
pBBR1MCS2_START | Kmr; oriRK2 mobRK2 | 57 |
pMAMV240 | Kmr; a 2.1-kb PCR fragment containing the mcpP gene and its promoter region was cloned into the HindIII/XbaI sites of pBBR1MCS2-START | This work |
Ap, ampicillin; Cm, chloramphenicol; Km, kanamycin; Rif, rifampin; Sm, streptomycin.