TABLE 2.
Thermodynamic parameters of ligand binding derived from the microcalorimetric titration of the McpP LBD with different ligandsa
| Ligand | KA (M−1) | KD (μM) | ΔH (kcal/mol) | nb |
|---|---|---|---|---|
| Acetate | (2.9 ± 0.4) × 104 | 34 ± 5 | −5.11 ± 3.1 | 0.77 ± 0.4 |
| Propionate | (2.9 ± 0.3) × 104 | 34 ± 4 | −5.35 ± 3.3 | 1.37 ± 0.6 |
| Pyruvate | (2.6 ± 0.2) × 104 | 39 ± 3 | −9.94 ± 2.6 | 0.89 ± 0.2 |
| l-Lactate | (9.3 ± 1) × 103 | 107 ± 11 | −5.20 ± 4.1 | 1.26 ± 0.8 |
a
Data are means and standard deviations from three experiments.
b
Stoichiometry of binding of ligands to the McpP LBD.