FIG 3.

Representative images of spectral unmixing of red and green signals in mice after oral administration of bioluminescent bacteria. (A) Multispectral acquisition of red- and green-emitting Lb. plantarum. Groups of mice were fed with Lp-CBRluc (CBR), Lp-CBGluc (CBG), or a mixture of both strains (50/50; Mix). Images were taken using a set of 20-nm filter step emission filters ranging from 500 nm to 620 nm. (B) Resulting unmixed images used for quantification and recomposition of the two different luciferases in false colors (red for CBR, green for CBG, and yellow for both luciferases).