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. 2015 Jul 21;81(16):5363–5374. doi: 10.1128/AEM.01221-15

FIG 2.

FIG 2

RT-PCR of adjacent genes for detecting polycistronic messages. (A) Schematic of gene cluster SGO_2103 to SGO_2106 with gene junction primers indicated by black arrows (not to scale). (B) Amplicons of SGO_2103 to SGO_2106 from RNA-Seq2 samples using gene junction primers: 1-kb DNA ladder, KS1 cDNA, AbpA− cDNA, and genomic (g) CH1 DNA (positive control). A robust amplicon was observed only at the SGO_2104-SGO_2105 gene junction in KS1 cDNA. (C) Schematic of redox gene cluster SGO_1173 to SGO_1180 with gene junction primers indicated by black arrows (not to scale). (D) One-kilobase DNA ladder, no-template control (NTC, negative control), KS1 cDNA, and CH1 genomic DNA (positive control). The cDNAs between the seven gene junctions from SGO_1173 to SGO_1180 were amplified using gene junction primers. Amplicons were found between all genes except for SGO_1175 and SGO_1176, suggesting two polycistronic messages.