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. 2015 Jul 21;81(16):5593–5603. doi: 10.1128/AEM.03987-14

TABLE 2.

Primers used in this work

Primer Sequence (5′-3′)a Restriction site
GBNdF1 5′-CAAATTCCATATGAGATCCCTCACTTGGG-3′ NdeI
GBNoR1 5′-CATAACTTGCGGCCGCATACTTCATTCACAGC-3′ NotI
GBEco2 5′-CGGAATTCAGAAGCTGAACATTG-3′ EcoRI
GBXhR2 5′-GCAGTACAAGCTCAAGGACG-3′
GBXbF1 5′-TACAAATCTAGAAAGCACTATGG-3′ XbaI
GBEco1 5′-GTCGAATTCTGTGATCGGGATG-3′ EcoRI
GBM1F1 5′-GATTGCTGCTCCGAATTTCC-3′
GBM1R1 5′-GCTATCCGTCTCACTCTTAC-3′
GBRTR1 5′-TGGTGTGATCGGGATGTCG-3′
a

Restriction sites are underlined.