TABLE 2.
Primer | Sequence (5′-3′)a | Restriction site |
---|---|---|
GBNdF1 | 5′-CAAATTCCATATGAGATCCCTCACTTGGG-3′ | NdeI |
GBNoR1 | 5′-CATAACTTGCGGCCGCATACTTCATTCACAGC-3′ | NotI |
GBEco2 | 5′-CGGAATTCAGAAGCTGAACATTG-3′ | EcoRI |
GBXhR2 | 5′-GCAGTACAAGCTCAAGGACG-3′ | |
GBXbF1 | 5′-TACAAATCTAGAAAGCACTATGG-3′ | XbaI |
GBEco1 | 5′-GTCGAATTCTGTGATCGGGATG-3′ | EcoRI |
GBM1F1 | 5′-GATTGCTGCTCCGAATTTCC-3′ | |
GBM1R1 | 5′-GCTATCCGTCTCACTCTTAC-3′ | |
GBRTR1 | 5′-TGGTGTGATCGGGATGTCG-3′ |
Restriction sites are underlined.