TABLE 5.
A. niger GBase sequence retrieved by MS/MS analysis
| Sequence type | Sequence |
|---|---|
| Peptide fragments retrieved by MS/MS analysisa | DLGGWYGRK |
| QMAGTSYNTR | |
| SPVTPASSEYGSK | |
| LVTLGGDHSVALPALR | |
| SKLVTLGGDHSVALPALR | |
| DCGDIPITPFDNGLAER | |
| LNIVGADIVEVSPSYDNK | |
| ALYQIYQKPITVLHFDAHLDTWNPVR | |
| Retrieved GBase sequence from A. niger genomeb | MRSLTWAACLALAGTAFAHAHHDHEEVPEHVREELLRKWDQEFTFSGIASFAHLKPVKCLIEPDERYDIAVIGAPFDTAVSYRPGARFGPRSIRAASARQMAGTSYNTRAGINPYASWATVKDCGDIPITPFDNGVAERQMYEAFLELGSRPALTSADSKYGTKGISAGKSKLVTLGGDHSVALPALRALYQIYQKPITVLHFDAHLDTWNPVRYSAYWTSEQAHFNHGSFFHKASREGLICNSTSAHAGLRTRLTGVDDSDYTNPGPEQGFMRIHADDIDDLGPMGIVNRIIERIGLDSDQPVYLSVDIDVLDPATAPGTGTPEPGGWTTREFIRILRGIEKLNIVGADIVEVSPSYDNKGETTALAAAQVAFEIITSMVKAGAGEELGGWYGRKVTPVEETVVVEEGEDPVEKKEAKDEL |
a
The eight peptide fragments are presented in order of frequency of their occurrence (highest to lowest).
b
The A. niger GBase sequence was retrieved (protein identifier, 55649; location, chr_4_2:3573655-3575104) by querying the A. niger genome database (http://genome.jgi-psf.org/Aspni5/Aspni5.home.html) with the AN7488.2 hypothetical protein of A. nidulans (see Materials and Methods). Peptide fragments identified through MS/MS analysis are highlighted in gray. The single underlining and double underlining indicate that the overlapping SKLVTLGGDHSVALPALR and LVTLGGDHSVALPALR sequences were found as peptide fragments; both are distinct from the subsequent fragment, ALYQIYQKPITVLHFDAHLDTWNPVR.