TABLE 1.

Primers used in this study^a

Primer name	Sequence (5′-3′)	Tab (°C)	Length (bp)
aroAqPCRFor	GGT ACT GCT ATG CGT TTG C	54.3	128
aroAqPCRRev	GCA CCA CCT TGT CTT AAA GC
pabBqPCRFor	CGC AAA TTG GAA CCG TAT CAG	50.2	154
pabBqPCRRev	CCC GTA ATT GAC CCA CCT G
folPqPCRFor	TTT CTG ATG GTG GAC AGT TTA C	51.5	149
folPqPCRRev	TCG TTC TGA TTC TTC AAG TTC G
rpsCqPCRFor	CAA GAC CTG GAA TAG TAA TCG G	50.1	198
rpsCqPCRRev	CAC GCT TCA TTG CTC TAC G
aroAstdFor	TTT TAT TAT CGG CGC AAA CC	55.0	457
aroAstdRev	AAT GGG GCC ATG ATG AGT AA
pabBstdFor	TAA CTG CGC ACC ATT TTC TG	52.0	468
pabBstdRev	CCA CAC CAT GCA TTT CTT CT
folPstdFor	AAA TGT CAC ACC GGA TTC GT	55.0	498
folPstdRev	CCG GGG TCA ATG ATT ATA CG
rpsCstdFor	TGG CGT TCT ACA TGG TAT GC	55.0	702
rpsCstdRev	TGC ACG AAA AGT GTG TAG GG
GmBtubfor	CCA TTC CCA CGT CTT CAC TT	55.0	151
GmBtubrev	GAC CAT GAC GTG GAT CAC AG
GmRFCfor	CTC AAA GCC ACC ACC TTG TT	55.0	294
GmRFCrev	CAA CGA TGA CAA GAC GGC TA

^aThe qPCR primers used in this study were designed using Beacon Designer software (version 7.2; Premier Biosoft International), and internal standard primers were created using Primer3 software (http://frodo.wi.mit.edu/).

^bT_a, annealing temperature.