TABLE 4.
Efficacy of asRNA silencers targeting lspA
| Antisense plasmid | Target region of lspA | Growth inhibition with IPTG | Reduction in mRNA levela |
Fold reduction in globomycin MICb | |||
|---|---|---|---|---|---|---|---|
| ribF | ileS | lspA | ispH | ||||
| pHNLA2 | −45 to +55 | Yes | − | + | + | − | 4 |
| pHNLA3 | +1 to +100 | Yes | − | + | + | − | 4 |
| pHNLA4 | +21 to +138 | Yes (slight) | − | − | − | − | 0 |
| pHNLA5 | +51 to +151 | Yes (slight) | − | − | − | − | 0 |
a
Only mRNA significantly reduced (n = 3; P < 0.05) in a dose-dependent manner are considered. −, no reduction; +, reduction.
b
Fold reduction in MIC at 0.2 mM IPTG was determined relative to the level of uninduced cultures in two repeated assays. Growth was scored visually.