Maximum-likelihood tree of the spliced sequences of the 7 loci (3,036 bp) for the 80 strains. Seventy Cronobacter strains isolated from PIF or an infant formula production factory, 4 reference strains (C. sakazakii ATCC BAA-894, C. sakazakii ATCC 29004, C. sakazakii ATCC 29544, and C. sakazakii ATCC 12868), and 6 type strains (C. malonaticus CDC 105877T, C. dublinensis LMG 23823T, C. turicensis LMG 23827T, C. universalis NCTC 9529T, C. condimenti LMG 26250T, and C. muytjensii ATCC 51329T) were included. The tree was obtained using MEGA, version 6.0, with 1,000 bootstrap replicates.