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. 2015 Jul 8;2015:217258. doi: 10.1155/2015/217258

Figure 1.

Figure 1

Time-course of neuronal PC12 cells death following NGF deprivation. (a) β-III tubulin immunostaining on PC12(615) cells differentiated with NGF (10 ng/mL) for 6 days. (b) MTT assay on neuronal PC12 cells NGF-deprived for the indicated times. Data, expressed as percent of CTR-NGF at the indicated time points, are the mean ± SEM of five independent experiments, each performed in triplicate. CTR-NGF did not change at each time point. ∗ p ≤ 0.05, ∗∗ p ≤ 0.01 versus CTR-NGF (ANOVA and Dunnett's multiple comparisons test). (c) Representative images of neuronal PC12 cells, CTR, or NGF-deprived for 72 h.