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. 2015 Jul 21;10(7):e0133190. doi: 10.1371/journal.pone.0133190

Fig 6. ApoL9 interacts with prohibitins.

Fig 6

A. Coomassie blue-stained gel of ApoL9b co-immunoprecipitation products. Samples were co-immunoprecipitated with an anti-FLAG antibody from 293T cells that were transfected with the pTM943 empty vector or with MK65 and MK66 expressing N- or C-terminally FLAG-tagged ApoL9b, respectively. Long arrows point to the tagged ApoL9 bands and short arrows, numbered 1 and 2 indicate co-immunoprecipitated products. Note that bands 1 and 2 were also visible but at a lower intensity in the ApoL9b-FLAG sample (right lane). L is the protein molecular weight ladder. B. Proteins corresponding to bands 1 and 2, as identified by mass spectrometry in mouse (Neuro-2A) and human (293T) cells. C. Co-immunoprecipitation of ApoL9 with Phb1 and Phb2. Western blot detection of FLAG-tagged ApoL9b after immunoprecipitation of endogenous Phb1 or Phb2 proteins. Lysates were prepared from 293T cells transfected with the empty pTM943 vector, with pTM943 derivatives expressing ApoL9b-FLAG or FLAG-ApoL9b and, as a negative control, with plasmid pMK86 expressing a N-terminally FLAG-tagged L* protein of TMEV. The upper panel shows FLAG detection in cell lysates corresponding to 5% of the input used for immunoprecipitations. Central and lower panels show FLAG detection after immunoprecipitation of Phb1 and Phb2, respectively.