Table 2. Recombinant lentiviral vectors used in the study.
| Plasmid/Virus 1 | Parental | Characteristics 2 |
|---|---|---|
| (p)TM942 | pCCLsin | Lentiviral vector carrying: PGK->MCS-IRES-mCherry |
| (p)TM943 | pCCLsin | Lentiviral vector carrying: CMV->MCS |
| (p)TM944 | pCCLsin | Lentiviral vector carrying: CMV->MCS-IRES-eGFP |
| (p)TM945 | pCCLsin | Lentiviral vector carrying: CMV->MCS-IRES-mCherry |
| (p)MK44 | pTM942 | PGK-> ApoL9b-IRES-mCherry |
| (p)MK65 | pTM943 | CMV-> FLAG-ApoL9b3 |
| (p)MK66 | pTM943 | CMV-> ApoL9b-FLAG4 |
| (p)MK82 | pTM945 | CMV-> FLAG-ApoL9b-IRES-mCherry |
| (p)MK83 | pTM945 | CMV-> ApoL9b-FLAG-IRES-mCherry |
| (p)MK85 | pTM942 | PGK-> ApoL9a-IRES-mCherry |
| (p)MK86 | pTM943 | CMV-> FLAG-L*5 |
| (p)MK87 | pTM943 | CMV-> FLAG-ApoL9a3 |
| (p)MK88 | pTM943 | CMV->ApoL9a- FLAG4 |
| (p)MK89 | pTM942 | PGK-> ApoL9b D50 N-terminal residues-IRES-mCherry |
| (p)MK90 | pTM942 | PGK-> ApoL9a D50 N-terminal residues-IRES-mCherry |
| (p)MK95 | pTM942 | PGK-> ApoL9b D50 C-terminal residues-IRES-mCherry |
| (p)MK96 | pTM942 | PGK-> ApoL9a D50 C-terminal residues-IRES-mCherry |
| (p)MK101 | pTM942 | PGK-> ApoL9b with mutated BH3-IRES-mCherry |
| (p)MK102 | pTM942 | PGK-> ApoL9a with mutated BH3-IRES-mCherry |
| (p)MK117 | pLKO.1 | shRNA empty vector with E2-Crimson-coding region replacing PuroR |
| (p)MK118 | pMK117 | shRNA targeting Phb1 coding region and co-expression E2-Crimson |
| (p)MK119 | pMK117 | shRNA targeting Phb1 coding region and co-expression E2-Crimson |
| (p)MK120 | pMK117 | shRNA targeting Phb2 coding region and co-expression E2-Crimson |
| (p)MK121 | pMK117 | shRNA targeting Phb2 3'UTR and co-expression E2-Crimson |
1. the names of plasmids used to produce the listed lentiviral vectors contain an extra p (e.g. pMK44 for lentivirus MK44)
2. pCCLsin = pCCLsin.PPT.hPGK.GFP.pre
3. Sequence of the FLAG-Apol9 junction is: MG-DYKDDDDK-GS-MASS… where GS is a linker and MASS are the N-terminal 4 residues of ApoL9.
4. Sequence of the Apol9-FLAG junction is: …YKTI-GS-DYKDDDDK where GS is a linker and YKTI are the C-terminal 4 residues of ApoL9.
5. L* = L star protein of Theiler’s virus; PGK = human phosphoglycerate kinase promoter; CMV = cytomegalovirus promoter; MCS = multi-cloning site; eGFP = enhanced GFP; PuroR = puromycin resistance; Δ = deletion