Table 3. primers used for gene expression analysis by RT-qPCR.
| Gene/virus 1 | Primer | Sequence 5’→ 3’ | Ref. plasmid 2 |
|---|---|---|---|
| TMEV (s) | TM348 | GAA CGT CAG CAT TTT CCG GC | pTM410 |
| TMEV (as) | TM349 | GGT GTG AAG AGC GGC AAG TG | pTM410 |
| β (s) | TM421 | AGA GGG AAA TCG TGC GTG AC | pTM793 |
| β (as) | TM422 | CAA TAG TGA TGA CCT GGC CGT | pTM793 |
| ApoL9b (s) | TM1479 | GTA GCT AGG ATT GTC AAC AAG A | pMK72 |
| ApoL9b (as) | TM1513 | CAG AGG GGT TCC TTC CAG CC | pMK72 |
| Oasl2 (s) | TM638 | GGA TGC CTG GGA GAG AAT CG | pCS40 |
| Oasl2 (as) | TM639 | TCG CCT GCT CTT CGA AAC TG | pCS40 |
| ApoL9a (s) | TM1464 | GTG GAT AGG ATT GCC AGC AAG | pMK68 |
| ApoL9a (as) | TM1465 | AGA GGG GTT CCT TTC AGA CTG | pMK68 |
1. (s)sense and (as) antisense
2. Ref. plasmids: reference plasmids containing the corresponding amplicons and used for quantitative PCR.