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. 2015 Jul 22;35(29):10390–10401. doi: 10.1523/JNEUROSCI.0546-15.2015

Figure 6.

Figure 6.

Effect of AS252424 and human recombinant active-PI3Kγ protein on neuroinflammation associated with SBI 24 h after surgery. Representative images are shown of Western blot assay for mast cell tryptase, CD3, MPO, E-selectin, and IL-1β level within peri-resection brain tissues (A). Compared with the vehicle-treated SBI, AS 252424 significantly reduced levels of mast cell tryptase (B), T-cell associated with CD 3 level (C), neutrophils associated MPO level (D), cell-adhesion molecule E-selectin (E), and IL-1β cytokine (F) elevation by SBI. Human recombinant active-PI3Kγ partially reversed the protective effect of AS252424 against neuroinflammation (n = 6/group). One-way ANOVA followed by Student–Newman–Keuls post hoc tests were used. B, **p < 0.001 versus sham; &&p < 0.001 versus SBI + AS252424 10 mg/kg; #p = 0.016 versus SBI + vehicle. C, **p < 0.001 versus sham; &&p = 0.003 versus SBI + AS252424 10 mg/kg; &p = 0.019 versus SBI + AS252424 10 mg/kg. D, *p = 0.023 versus sham; **p < 0.001 versus sham; &&p = 0.004 versus SBI + AS252424 10 mg/kg. E, *p = 0.039 versus sham; **p = 0.002 versus sham; &&p = 0.008 versus SBI + AS252424 10 mg/kg. F, *p = 0.029 versus sham; **p < 0.001 versus sham; &p = 0.026 versus SBI + AS252424 10 mg/kg; &&p = 0.001 versus SBI + AS252424 10 mg/kg.