Figure 11.

Impaired endosome-to-Golgi retrieval of Lamp2a in NLT cells expressing VPS35-D620N. Decreased Lamp2a in NLT cells expressing PD-linked VPS35 mutant (D620N). Transfected NLT cells were fixed and immunostained using anti-Lamp2a antibody. A, Representative images. Scale bar, 10 μm. B, Pearson's correlation coefficient of VPS35/VPS35-D620N-GFP with Lamp2a. C, Line profile intensity of Lamp2a from the dotted line in A. D, Quantification of Lamp2a optical density. The values of mean ± SEM (n = 10; *p <0.05) were presented.