Figure 2.

Enhanced surface permeability in rwa2. (A) Water loss from detached leaves of wild type, rwa2-1 and rwa2-3 plants. Rosette leaves were detached from 4-weeks old plants and incubated on the lab bench. Water loss was determined as percentage weight loss compared to time zero. The data are average ± SD of four leaves per time point. (B) Water relations of wild type and rwa2-3 leaves measured by gas exchange analysis. Values are means ± SD of four leaves from individual plants. (C) The aperture of guard cells in response to exogenous ABA and drought. The apertures of guard cells were measured in epidermal peels from leaves treated with 10 μM ABA (left) or in epidermal peels from detached leaves that had been incubated on the bench for 1 h (right) (from plants incubated at high humidity over-night). The data is average of 40 guard cells ± SD. The experiment was repeated three times with similar result. An asterisk indicate a statistically significance difference from the wild type by Student's t-test (P < 0.05).