Figure 3.

Trichome integrity is altered in rwa2. (A) Macroscopic appearance of 3-weeks old wild type, rwa2-1 and rwa2-3 grown in growth chamber with 12 h dark/light cycle. (B) Trichomes of the wild type (right) and rwa2-3 (left). The arrow indicates a collapsed trichome on rwa2-3. The scale bar equals 0.5 mm. (C) The percentage of “normal looking” trichomes [as depicted in (B left)] on the leaves of wild type, rwa2-1 and rwa2-3. Leaves were destained in ethanol followed by quantification of the total number of trichomes and the number of collapsed trichomes when viewed under the microscope. The data is the average of three independent experiments ± SD. The number of non-collapsed trichomes on rwa2-1 and rwa2-3 leaves are significant different from wild type as tested by Student's t-test (P < 0.05) as indicated by the asterisks. (D) Trichome birefringence of the wild type (left) and rwa2-3 (right) leaves. Scale bar equals 1 mm.