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. 2015 Jul 22;6:550. doi: 10.3389/fpls.2015.00550

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Copyright © 2015 Nafisi, Stranne, Fimognari, Atwell, Martens, Pedas, Hansen, Nawrath, Scheller, Kliebenstein and Sakuragi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Epicuticular wax and cutin composition analysis of leaves of 4-weeks old wild type, rwa2-1 and rwa2-3 plants. (A) Composition of epicuticular waxes extracted by dipping in chloroform and derivatized with BSTFA. (B) Composition of depolymerized residual-bound lipids extracted extensively with methanol/chloroform followed by NaOMe-catalyzed acetylation. Values are means of at least five independent samples and the error bars represent the standard deviations. An asterisk indicate a statistically significance difference from the wild type by Student's t-test (P < 0.05).