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. 2015 Jul 22;6:561. doi: 10.3389/fpls.2015.00561

Table 1.

Analysis of fruit formation as a result of hand pollinations performed at different stages of N. tabacum flower development.

Flower developmental stages Length of floral bud (mm) Number of flower buds pollinated Presence of fruit Amount of fruits formed (%) Average fruit weight (mg)∗∗
4 16–19 15 No 0 (0%) -
5 20–21 15 No 0 (0%) -
6 22–27 16 No 0 (0%) -
Early 7 28–33 09 No 0 (0%) -
Late 7 34–38 11 Yes 11 (100%) 5.88 ± 2.9a
8 39–42 17 Yes 17 (100%) 9.20 ± 4.9ab
9 43–44 12 Yes 12 (100%) 12.13 ± 4.7ab
10 45–46 13 Yes 13 (100%) 14.13 ± 7.6b
11 47 12 Yes 12 (100%) 14.75 ± 7.8b

*Data originally published by Koltunow et al. (1990) and used in the present work to establish the stages of tobacco flower development.

**Different letters indicate significant differences of fruit weight. Statistical analyses were performed using analysis of variance (ANOVA) and Contrast comparison with p ≤ 0.05.