Fig 6. Transglycosylation of deglycosylated RNase B by Endo-CC1N180H and End-CC1N180Q at various SGP concentration.
SDS-PAGE analysis of transglycosylation reaction mixtures that contained deglycosylated RNase B, purified Endo-CC1 mutant (Endo-CC1N180H or EndoCC1N180Q) and different amounts of complex type oligosaccharide derived from SGP: 1 h incubation (A) and 12 h incubation (B). Amount of SGP used in the reaction mixture (μg): Lane 1, 62.5; lane 2, 125; lane 3, 250; lane 4, 500; lane 5, 1000. Note that in lanes 5, mobility shifts were seen likely due to a large amount of SGP included in the samples.